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Construction of Cyclic Cell-Penetrating Peptides for Enhanced Penetration of Biological Barriers

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Published on: September 19, 2022

Fluorescent labeling of peptides on solid phase.

Alan R Katritzky¹, Megumi Yoshioka, Tamari Narindoshvili

¹University of Florida, Gainesville, FL 32611-7200, USA. katritzky@chem.ufl.edu

Organic & Biomolecular Chemistry

|November 29, 2008

Summary

This summary is machine-generated.

Researchers developed a new method for synthesizing fluorescent lysine derivatives. This benzotriazole methodology enables efficient microwave-enhanced solid-phase fluorescent labeling of peptides for various applications.

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Detection of Protease Activity by Fluorescent Peptide Zymography

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Published on: January 20, 2019

Area of Science:

Organic Chemistry
Biochemistry
Peptide Chemistry

Background:

Fluorescent labeling of peptides is crucial for biochemical assays and diagnostics.
Existing methods for synthesizing fluorescently labeled amino acids can be complex and inefficient.
The development of novel reagents and methodologies is needed to streamline peptide labeling.

Purpose of the Study:

To develop a convenient and efficient method for preparing N(alpha)-Fmoc-N(epsilon)-[(7-methoxycoumarin-4-yl)acetyl]-L-lysine (N(alpha)-Fmoc-L-Lys(Mca)-OH).
To synthesize novel N-acylbenzotriazole derivatives for peptide labeling.
To demonstrate the utility of these compounds in microwave-enhanced solid-phase fluorescent labeling of peptides.

Main Methods:

Preparation of N(alpha)-Fmoc-L-Lys(Mca)-OH using benzotriazole methodology.
Synthesis of various N-acylbenzotriazoles, including Mca-Bt, N(alpha)-Fmoc-L-Lys(Mca)-Bt, Cc-Bt, N(alpha)-Fmoc-L-Lys(Cc)-Bt, and N(alpha)-(Cc)-L-Lys(Fmoc)-Bt.
Application of synthesized compounds in microwave-enhanced solid-phase fluorescent peptide labeling.

Main Results:

N(alpha)-Fmoc-L-Lys(Mca)-OH was synthesized in 52% yield over two steps.
A series of N-acylbenzotriazoles were successfully prepared.
Efficient microwave-enhanced solid-phase fluorescent labeling of peptides was achieved using the developed reagents.

Conclusions:

The benzotriazole methodology provides a convenient route for synthesizing fluorescent lysine derivatives.
The novel N-acylbenzotriazole reagents facilitate efficient solid-phase fluorescent labeling of peptides.
This approach offers a streamlined method for preparing fluorescently labeled peptides for research and diagnostic purposes.