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Updated: Jun 27, 2026

Protocol for Plasmodium falciparum Infections in Mosquitoes and Infection Phenotype Determination
Published on: July 4, 2007
[Malaria in Spain: entomological aspects and future outlook]
Rubén Bueno Marí1, Ricardo Jiménez Peydró
1Laboratorio de Entomología y Control de Plagas, Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universidad de Valencia, Valencia. ruben.bueno@uv.es
Abstract:
Malaria was officially eradicated in Spain in 1964. However, at the present time, hundreds of imported cases are registered in our country each year. In this context, the study of the vector is seen to be highly significant in order to infer possible transmission scenarios, whether of a sporadic or a regular nature. Although the socio-economic level of the country does not appear to foreshadow the possible re-emergence of the disease in the short and medium term, the presence of well-established populations of anophelini and plasmodium gametocytes circulating in a certain percentage of the human population does appear to warrant the continuation of the current status of epidemiological surveillance. Moreover, the globalisation of markets and the emerging process of climate change could enable the colonisation of our territory by part of the Anopheles species that transmit human plasmodiosis in tropical and subtropical regions. In order to obtain a more thorough knowledge of the range of fauna, spatial distribution and bioecology of the anopheline Culicoides, a number of intensive larval samplings were taken in the Community of Valencia, a region with sufficient surface water heterogeneity and historical data of malaria prevalence to substantiate the decision to choose it for this study. Five species of the Anopheles genus, with varying degrees of impact in the dissemination of the disease, were identified.
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