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Microarray analysis of the microflora of root caries in elderly.
D Preza1, I Olsen, T Willumsen
1Institute of Oral Biology, University of Oslo, Norway. doritap@odont.uio.no
Summary
This study reveals distinct bacterial profiles in elderly individuals with root caries (RC). Certain bacteria, like Lactobacillus and Pseudoramibacter, are linked to RC, suggesting they are key pathogens beyond Actinomyces and Streptococcus mutans.
Area of Science:
- Microbiology
- Geriatric Dentistry
- Oral Health
Background:
- Root caries (RC) is a significant oral health issue in the elderly.
- Understanding the specific bacterial communities associated with RC is crucial for targeted prevention and treatment.
- Previous studies have indicated a role for specific bacteria, but a comprehensive profile in the elderly population is needed.
Purpose of the Study:
- To define the bacterial profiles of healthy root surfaces and root caries in elderly individuals.
- To identify bacterial species associated with root caries in this demographic.
- To compare bacterial diversity between healthy controls and patients with root caries.
Main Methods:
- Utilized a novel 16S rRNA-based microarray with probes for over 300 bacterial species.
- Collected supragingival plaque samples from 20 healthy elderly subjects (Controls) and 21 elderly subjects with root caries (Patients).
- Analyzed plaque from healthy roots, carious roots, and carious dentin.
Main Results:
- Detected 179 bacterial species and species groups in total.
- Observed higher bacterial diversity in healthy controls compared to patients with root caries.
- Found Lactobacillus casei/paracasei/rhamnosus and Pseudoramibacter alactolyticus strongly associated with most root caries samples.
- Streptococcus mutans was more frequent in infected dentin, but not significantly.
- Actinomyces species were more prevalent in controls.
Conclusions:
- Bacterial profiles differ significantly between healthy elderly individuals and those with root caries.
- Lactobacillus and Pseudoramibacter species appear to be key pathogens in elderly root caries.
- The findings suggest that bacteria other than Actinomyces and Streptococcus mutans play a significant role in the pathogenesis of root caries in the elderly.
- Results align with previous 16S rRNA gene sequencing studies.
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