Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Real time quantitative amplification detection on a microarray: towards high multiplex quantitative PCR.

Lab on a chip·2012

Same author

Rapid genotyping of human papillomavirus by post-PCR array-based hybridization techniques.

Journal of clinical microbiology·2011

Same author

Immobilization of oligonucleotides with homo-oligomer tails onto amine-functionalized solid substrates and the effects on hybridization.

Analytical chemistry·2010

Same author

Fluid dynamical analysis of the distribution of ink jet printed biomolecules in microarray substrates for genotyping applications.

Biomicrofluidics·2009

Same author

Mass transfer effects on DNA hybridization in a flow-through microarray.

Journal of biotechnology·2008

Same author

Quantitative analysis of DNA hybridization in a flowthrough microarray for molecular testing.

Analytical biochemistry·2008

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

Quality control of inkjet technology for DNA microarray fabrication.

Anke Pierik¹, Frits Dijksman, Adrie Raaijmakers

¹Philips Research Europe, Eindhoven, The Netherlands.

Biotechnology Journal

|November 29, 2008

Summary

This study analyzed inkjet printing for DNA microarray manufacturing, identifying causes of jetting failures. The process achieved a 99.9% yield, demonstrating its robustness for diagnostic test development.

More Related Videos

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

DNA Microarrays: Sample Quality Control, Array Hybridization and Scanning

Published on: March 15, 2011

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries

Published on: August 12, 2019

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Experimental Protocol for Detecting Cyanobacteria in Liquid and Solid Samples with an Antibody Microarray Chip

Published on: February 7, 2017

Area of Science:

Biotechnology
Manufacturing Engineering
Materials Science

Background:

High-quality DNA microarray manufacturing is critical for diagnostic applications.
Inkjet printing is a common method for fabricating microarrays.
Understanding and mitigating process failures is essential for reliable production.

Purpose of the Study:

To investigate failure modes in inkjet printing for low-density DNA microarray manufacturing.
To analyze the frequency and causes of jetting failures during microarray production.
To assess the overall yield and robustness of the automated manufacturing process.

Main Methods:

Utilized a single nozzle inkjet spotter equipped with dual optical imaging systems.
Monitored droplet flight paths in real-time to detect emission failures.
Analyzed over 1.3 million droplets to identify failure patterns and causes.

Main Results:

96.2% of substrates were manufactured without system or jetting failures.
Droplet emission failures were correctly identified in 1.6% of substrates.
Imaging system failures occurred in 2.2% of substrates, while droplet emission was correct.
Undetected droplet emission failures occurred in only 0.1% of substrates.
The overall manufacturing yield was determined to be 99.9%.

Conclusions:

The automated inkjet printing process with real-time monitoring is highly reliable for DNA microarray manufacturing.
The system effectively detects and allows for correction of most jetting failures.
A 99.9% yield indicates the process is suitable for prototyping and potential diagnostic applications.