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Updated: Jun 27, 2026

Broth Microdilution In Vitro Screening: An Easy and Fast Method to Detect New Antifungal Compounds
Published on: February 14, 2018
Comparative evaluation of Trichosporon asahii susceptibility using ASTY colorimetric microdilution and CLSI M27-A2
Ayse Kalkanci1, Nanthawan Mekha, Natteewan Poonwan
1Gazi University Faculty of Medicine, Department of Microbiology, Besevler, Ankara, 06500, Turkey.
Abstract:
The in vitro activity ofAMPH-B, 5-FC, FLCZ, MCZ, ITCZ, and VRCZ against 50 isolates of T. asahii was determined using CLSI M27-A2 microdilution and ASTY colorimetric methods. Observed agreement ranged from 96 to 100% according to the drug. Overall, the agreement between two methods was 97.7%. The ASTY colorimetric method was thus determined to be comparable to the CLSI reference method when testing the susceptibility of T. asahii to a variety of antifungal agents.
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