Angiotensin II receptor 1 involved in the central pressor response induced by interleukin-1 beta in the paraventricular nucleus
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Updated: Jun 27, 2026

Receptor Autoradiography Protocol for the Localized Visualization of Angiotensin II Receptors
Published on: June 7, 2016
Yang Lu1, Jianguang Chen, Xiaohua Yin
1Department of Physiology, School of Basic Medical Sciences, Jilin University, Jilin, China.
Objective:
To investigate the possible participation of angiotensin II receptor 1 (AT(1) receptor) in pressor response induced by IL-1beta in the paraventricular nucleus (PVN) of hypothalamus.
Methods:
The experiments were performed on adult Wistar rats. Mean arterial pressure (MAP) were recorded during microinjection of different doses of IL-1beta, IL-1beta together with Losartan, an AT1 receptor antagonist, or Ang II pre-treated with IL-1beta into PVN. Rats microinjected with saline were served as controls in all experiments.
Results:
Microinjection of IL-1beta (0.1 or 0.2 ng) resulted in increases in blood pressure in a dose-dependent manner, and the pressor effect caused by administration of IL-1beta (0.2 ng) could be attenuated by pre-treatment with AT(1) receptor antagonist (100 ng Losartan). A subpressor dose of IL-1beta (0.05 ng) could augment the pressor response induced by microinjection of Ang II (10 ng).
Discussion:
The present results provide support that central infusion of IL-1beta increases blood pressure and the pressor response requires stimulation of AT(1) receptors. It is also shown that IL-1beta has the effect of increasing the magnitude of pressor response induced by Ang II in PVN.
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