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Alterations in calcium regulatory protein expression in patients with preserved left ventricle systolic function and

Przemyslaw Leszek1, Malgorzata Szperl, Anna Klisiewicz

  • 1National Institute of Cardiology, Warszawa, Poland. przemyslaw.leszek@ikard.pl

Journal of Cardiac Failure
|December 2, 2008
PubMed

Insights

In mitral valve stenosis, reduced SERCA2 expression in the left ventricle is linked to neurohumoral activation, impacting calcium handling despite preserved heart function.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Biochemistry

Background:

  • Heart failure involves altered calcium homeostasis proteins, affecting contractility and relaxation.
  • Gene expression regulation in heart failure is not fully understood.
  • Mitral valve stenosis (MVS) models show increased neurohumoral/cytokine (N/C) activation with preserved global left ventricular (LV) function.

Purpose of the Study:

  • To assess calcium regulatory protein expression in LV myocardium of MVS patients with preserved global function and elevated N/C activation.
  • To investigate the relationship between N/C activation markers and calcium handling proteins in MVS.

Main Methods:

  • Evaluated plasma N/C activation in 27 MVS patients.
  • Assessed expression of L-type channel, SERCA2, NCX, calsequestrin, and phospholamban in LV myocardium via Western Blot.
  • Compared MVS patients with non-failing hearts (NFH).

Main Results:

  • SERCA2 and NCX expression varied significantly among MVS patients.
  • SERCA2 expression was significantly reduced by 15% compared to NFH.
  • SERCA2 negatively correlated with BNP; NCX positively correlated with noradrenaline.

Conclusions:

  • MVS patients exhibit LV remodeling with preserved global function.
  • Neurohumoral factors like BNP and noradrenaline may influence Ca2+ handling protein expression in MVS, beyond LV load and wall stress.
Abstract

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