Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Studies on EDTA-dependent pseudoneutropenia].

S Kobayashi1, K Seki, M Yamaguchi

  • 1Division of Clinical Laboratory, Kanagawa Cancer Center.

[Rinsho Ketsueki] the Japanese Journal of Clinical Hematology
|March 1, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mode-selective optical packet switching in mode-division multiplexing networks.

Optics express·2015
Same author

Disseminated Cunninghamella bertholletiae infection with septic pulmonary embolism after allogeneic bone marrow transplantation.

Transplant infectious disease : an official journal of the Transplantation Society·2014
Same author

Ultra-broad band, low power, highly efficient coherent wavelength conversion in quantum dot SOA.

Optics express·2012
Same author

A prognostic score with pretransplant serum ferritin and disease status predicts outcome following reduced-intensity SCT.

Bone marrow transplantation·2011
Same author

Secondary solid tumors after allogeneic hematopoietic SCT in Japan.

Bone marrow transplantation·2011
Same author

Pre-SCT serum ferritin is a prognostic factor in adult AML, but not ALL.

Bone marrow transplantation·2010

EDTA-dependent pseudoleukocytopenia, a false low white blood cell count, results from neutrophil aggregation. This phenomenon is reversible and linked to IgM antibodies, but its exact mechanism remains unclear.

Area of Science:

  • Hematology
  • Clinical Pathology

Context:

  • EDTA-dependent pseudoleukocytopenia is a rare laboratory finding.
  • Observed in seven patients between September 1989 and March 1990.

Purpose:

  • To investigate the cause of EDTA-dependent pseudoleukocytopenia.
  • To characterize the neutrophil aggregation phenomenon.

Summary:

  • Pseudoleukocytopenia resulted from neutrophil aggregation, not other blood cells.
  • Aggregation peaked 30-180 minutes post-venipuncture at room temperature.
  • Aggregates dispersed upon warming to 37°C and were dependent on EDTA concentration.
  • Aggregation was absent with citrate or heparin anticoagulants.
  • Mercaptoethanol test and immunocytochemistry suggested a role for IgM antibodies.

Related Experiment Videos

  • Four patients had hepatic disorders, but the mechanism remains uncertain.

    • Impact:

    • Highlights a potential artifact in complete blood counts.
    • Suggests a link between EDTA, IgM antibodies, and neutrophil behavior.
    • Underscores the importance of considering anticoagulant type and sample handling in hematological analysis.