Related Experiment Video
Updated: Jun 27, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Quantification of isradipine in human plasma using LC-MS/MS for pharmacokinetic and bioequivalence study
Jin-Hee Park1, Yoo-Sin Park, Si-Youn Rhim
1Department of Pharmacology & Institute of Biomedical Sciences, College of Medicine, Hanyang University, Seoul, South Korea.
Abstract:
A highly sensitive and rapid method for the analysis of isradipine in human plasma using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was developed. The procedure involves a simple liquid-liquid extraction of isradipine and amlodipine (IS, internal standard) with methyl-t-butyl ether after alkaline treatment and separation by RP-HPLC. Detection was performed by positive ion electrospray ionization (ESI) in multiple reaction monitoring (MRM) mode, monitoring the transitions m/z 372.1-->m/z 312.2 and m/z 408.8-->m/z 237.9, for quantification of isradipine and IS, respectively. The standard calibration curves showed good linearity within the range of 10 to 5000 pg/mL (r(2)>or=0.9998). The lower limit of quantitation (LLOQ) was 10 pg/mL. The retention times of isradipine (0.81 min) and IS (0.65 min) suggested the potential for high throughput of the proposed method. In addition, no significant metabolic compounds were found to interfere with the analysis. This method offered good precision and accuracy and was successfully applied for the pharmacokinetic and bioequivalence studies of 5 mg of sustained-release isradipine in 24 healthy Korean volunteers.
Related Concept Videos
Measurement of Bioavailability: Pharmacodynamic Methods
Measurement of Bioavailability: Pharmacokinetic Methods
Bioavailability Study Design: Single Versus Multiple Dose Studies
Modified-Release Drug Delivery Systems: Bioavailability
Bioequivalence Data: Statistical Interpretation
Pharmacokinetic–Pharmacodynamic Relationship: Problems