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Related Concept Videos

Bone Markings01:26

Bone Markings

Bones have various surface features that help form joints and attach to other soft tissues. Depending on the function, bone markings are categorized into articulating projections, processes for attachment, depressions, and openings.
Articulating Projections
Articulating projections are found where two bones meet to form a joint. These structures are usually found at the ends of bones. The largest articulation is a rounded projection called the head, supported by a narrow neck at the ends of...
Design Example: Marking Boundaries of a Site Using a Compass01:12

Design Example: Marking Boundaries of a Site Using a Compass

Marking site boundaries using a compass is a precise surveying technique that ensures the accuracy of boundary delineation. The process begins by using provided site details, including the bearings and lengths of each boundary line. The initial step involves calculating latitudes and departures for all sides of the site. This computation verifies that the traverse is free of errors, ensuring a closed and accurate boundary.The process starts at a known point, such as Point A, which is often...
Margin of Error01:27

Margin of Error

The margin of error is also called the maximum error of an estimate. The margin of error is the maximum possible or expected difference between the observed sample parameter value and the actual population parameter value. For proportion, it is the maximum difference between the value of sample proportion obtained from the data and the true value of population proportion. As the true value of the population parameter is not known, the margin of error is calculated using the sample statistic.
Ellipses01:30

Ellipses

An ellipse is formed when a right circular cone is intersected by an inclined plane that does not cut through its base. This intersection yields a closed, symmetric curve characterized by distinctive geometric properties. Most notably, an ellipse is defined as the collection of all points in a plane for which the combined distances to two fixed points—called the foci—remain constant.The ellipse features two principal axes: the major and the minor axes. The major axis is the longest diameter,...
Line Loss01:10

Line Loss

The different configurations of source-load connections include wye (star) and delta connections. The relationship between line and phase voltages and currents varies depending on the configuration. When the source is supplying power, it is transmitted through the wires to the load, and during this transmission, some power is absorbed by the wires, leading to line loss.
Line loss impacts power delivery efficiency in a balanced three-phase circuit. The symmetry in such a circuit simplifies the...
Errors in Taping01:18

Errors in Taping

Errors in taping arise from multiple factors that can significantly impact measurement accuracy in surveying. Misalignment of the tape, often due to human error, is one primary source. A skilled rear tapeman, using a telescope, can help correct alignment by guiding the head tapeman; however, human limitations still lead to small inaccuracies. These errors may include misplacement of pins or inaccurate tape readings due to common visual confusions, such as mistaking a six for a nine. Such...

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Related Experiment Video

Updated: Jun 27, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica
06:05

Development of a Negative Selectable Marker for Entamoeba histolytica

Published on: December 12, 2010

An EZ mark to miss.

Lena Ho1, Gerald R Crabtree

  • 1Program in Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.

Cell Stem Cell
|December 2, 2008
PubMed
Summary
This summary is machine-generated.

Polycomb Repressive Complex II methyltransferase EZH2 is not essential for embryonic stem cell (ESC) derivation and self-renewal. EZH1 can unexpectedly compensate for EZH2 loss, challenging previous understandings of H3K27me3 marking in ESCs.

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Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
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Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

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Last Updated: Jun 27, 2026

Development of a Negative Selectable Marker for Entamoeba histolytica
06:05

Development of a Negative Selectable Marker for Entamoeba histolytica

Published on: December 12, 2010

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities
07:13

Multimodal Cross-Device and Marker-Free Co-Registration of Preclinical Imaging Modalities

Published on: October 27, 2023

Area of Science:

  • Epigenetics
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Polycomb Repressive Complex II (PRC2) proteins are crucial epigenetic regulators.
  • EZH2 (Enhancer of zeste homolog 2) is the catalytic subunit of PRC2, responsible for H3K27 trimethylation.
  • H3K27me3 is a key histone mark associated with gene silencing and embryonic stem cell (ESC) pluripotency.

Purpose of the Study:

  • To investigate the essentiality of EZH2 for ESC derivation and self-renewal.
  • To determine if other EZH proteins can compensate for EZH2 function in ESCs.
  • To re-evaluate the role of EZH2 in establishing H3K27me3 marks in ESCs.

Main Methods:

  • Generation of EZH2-deficient ESC lines.
  • Assessment of ESC derivation and self-renewal capacity in vitro.
  • Analysis of H3K27me3 levels and global gene expression in EZH2-deficient ESCs.
  • Comparison of EZH1 and EZH2 expression and function.

Main Results:

  • ESCs can be derived and maintained in the absence of EZH2.
  • EZH2-deficient ESCs exhibit normal self-renewal and pluripotency.
  • EZH1 is upregulated and compensates for the loss of EZH2's methyltransferase activity.
  • H3K27me3 marks are still established in EZH2-deficient ESCs, albeit with altered patterns.

Conclusions:

  • EZH2 is dispensable for ESC derivation and self-renewal.
  • EZH1 plays a compensatory role for EZH2 in maintaining H3K27me3 and pluripotency.
  • These findings challenge the long-held view of EZH2 as solely essential for H3K27me3 in ESCs.
  • The study highlights the functional redundancy within the EZH protein family.