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Preparation of Chitosan-based Injectable Hydrogels and Its Application in 3D Cell Culture
Published on: September 29, 2017
Synthesis and characterization of chitosan-based hydrogels
Qianzhu Li1, Dongzhi Yang, Guiping Ma
1State Key Laboratory of Chemical Resource Engineering and Key Lab of Beijing, City on Preparation and Processing of Novel Polymer Materials, Beijing University of Chemical Technology, Beijing 100029, PR China.
New biocompatible hydrogels made from chitosan-ethylene glycol acrylate methacrylate (CS-EGAMA) and polyethylene glycol diamethacrylate (PEGDMA) show pH sensitivity and no cytotoxicity. These advanced materials are suitable for various biomedical applications.
Area of Science:
- Biomaterials Science
- Polymer Chemistry
- Biotechnology
Background:
- Biocompatible hydrogels are crucial for advanced biomedical applications.
- Developing novel hydrogel formulations with tailored properties is an ongoing research area.
- Chitosan-based hydrogels offer unique advantages due to their inherent biocompatibility and biodegradability.
Purpose of the Study:
- To synthesize and characterize novel biocompatible hydrogels.
- To investigate the pH-sensitivity and cytotoxicity of the developed hydrogels.
- To evaluate the suitability of these hydrogels for bioapplications.
Main Methods:
- Photopolymerization was used to synthesize hydrogels from CS-EGAMA and PEGDMA.
- Characterization involved SEM, TGA, XRD, DSC, FTIR, swelling tests, and MTT assays.
- Cytotoxicity was assessed using L929 and SW1353 cell lines.
Main Results:
- The synthesized hydrogels exhibited pH-sensitive behavior.
- SEM, TGA, XRD, DSC, and FTIR confirmed the hydrogel structure and properties.
- No cytotoxicity was observed for L929 and SW1353 cell lines, indicating good biocompatibility.
Conclusions:
- The CS-EGAMA/PEGDMA hydrogels are biocompatible and pH-responsive.
- These hydrogels demonstrate significant potential for use in diverse biomedical applications.
- The study confirms the satisfactory performance of these composite hydrogels for bio-integration.
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