An enzyme cocktail for efficient protoplast formation in Aspergillus niger
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GENPLAT: an Automated Platform for Biomass Enzyme Discovery and Cocktail Optimization
Published on: October 24, 2011
Charissa de Bekker1, Ad Wiebenga, Guillermo Aguilar
1Microbiology, Institute of Biomembranes, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.
Journal of Microbiological Methods
|December 2, 2008
Abstract:
Novozym 234 has been traditionally used to prepare protoplasts for genetic transformation of fungi. Since it is no longer on the market, a new enzyme cocktail was defined to protoplast Aspergillus niger. The cocktail consists of lysing enzymes from Trichoderma harzianum, chitinase from Streptomyces griseus and beta-glucuronidase from Helix pomatia.
