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Modulation of phospholipase A2 activity in zymogen granule membranes by GTP[S]; evidence for GTP-binding protein
R P Rubin1, M Withiam-Leitch, S G Laychock
1Department of Pharmacology & Therapeutics, State University of New York, Buffalo 14214.
Biochemical and Biophysical Research Communications
|May 31, 1991
Abstract:
In membranes associated with purified pancreatic zymogen granules, GTP[S] elicited a concentration-dependent activation of phospholipase A2 (PLA2), which was converted to inhibition in the presence of added Ca2+. The GTP-binding protein inhibitor GDP[S] blocked both the stimulatory and inhibitory actions of GTP[S]. We conclude that in zymogen granule membranes GTP-binding proteins exert a dual regulation of PLA2 activity.