[Optimization of signaling pathways for bone regeneration]
Naoshi Ogata1, Ung-il Chung, Yuichi Tei
1The University of Tokyo, Faculty of Medicine, Division of Bone and Cartilage Regeneration, Japan.
Abstract:
We have developed an ES cell-based monitoring system for osteogenic differentiation and identified a potent combination of osteogenic genes. ES cells were isolated from mice carrying a transgene expressing GFP driven by the 2.3 kb fragment of rat type I collagen alpha1 promoter. We have screened cDNA libraries and combinations of major osteogenesis-related genes and identified that the combination of constitutively active activin receptor-like kinase 6 (caALK6) and runt-related transcription factor 2 (Runx2) was the minimal unit that induced GFP. These combinations of genes would be a potent and ideal tool for bone regeneration.
Related Concept Videos
Diversity in Cell Signaling Responses
Graded and Abrupt Responses
Some signaling systems generate...
Interactions Between Signaling Pathways
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Bone Remodeling and Repair
TGF - β Signaling Pathway
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...

