Related Experiment Video
Updated: Jun 27, 2026

Lentiviral Vector Preparation for Efficient Gene and MicroRNA Modulation of Peritoneal Cavity Tissue-Resident Macrophages In Vivo in Mice
Published on: February 16, 2024
Stable knockdown of microRNA in vivo by lentiviral vectors
Bernhard Gentner1, Giulia Schira, Alice Giustacchini
1San Raffaele Telethon Institute for Gene Therapy, Via Olgettina 58, Milan, Italy.
Abstract:
Studying microRNA function in vivo requires genetic strategies to generate loss-of-function phenotypes. We used lentiviral vectors to stably and specifically knock down microRNA by overexpressing microRNA target sequences from polymerase II promoters. These vectors effectively inhibited regulation of reporter constructs and natural microRNA targets. We used bone marrow reconstitution with hematopoietic stem cells stably overexpressing miR-223 target sequence to phenocopy the genetic miR-223 knockout mouse, indicating robust interference of microRNA function in vivo.
Related Concept Videos
Experimental RNAi
MicroRNAs
MicroRNAs
In-vitro Mutagenesis

