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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and the...

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A Multimodal Wide-Field Fourier-Transform Raman Microscope
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A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Implementation of maskless laser lithography using a Raman spectroscopy microprobe.

Barbara Codan1, Valter Sergo

  • 1CENMAT, Department of Materials and Natural Resources, University of Trieste, via Valerio 6/A, 34127 Trieste, Italy.

The Review of Scientific Instruments
|December 3, 2008
PubMed
Summary

A Raman microprobe, typically used for chemical analysis, can now perform maskless photolithography. This laser writing technique achieves high resolution, enabling precise patterning for applications like cell adhesion studies.

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Last Updated: Jun 27, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
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Area of Science:

  • Spectroscopy
  • Materials Science
  • Biotechnology

Background:

  • Raman spectroscopy microprobes are advanced instruments utilizing lasers for chemical analysis.
  • These microprobes often incorporate motorized stages for precise sample manipulation.
  • The interaction of focused laser light and stage movement is key to novel applications.

Purpose of the Study:

  • To describe a new application of Raman spectroscopy microprobe technology.
  • To demonstrate its capability in maskless photolithography (laser writing).
  • To evaluate the resolution and versatility of this laser writing method.

Main Methods:

  • Utilizing a Raman microprobe equipped with a motorized stage.
  • Coupling a focused laser light with precise stage movement for photolithography.
  • Testing the system by creating various patterns on photoresist.

Main Results:

  • Successful implementation of maskless photolithography using a Raman microprobe.
  • Achieved lateral resolution as small as 1 micrometer (µm).
  • Demonstrated versatility by producing different arrays of holes and protein patterns.

Conclusions:

  • Raman microprobe laser writing offers high resolution suitable for various applications.
  • This technique enables precise patterning of materials, including biological molecules.
  • The method is valuable for creating surfaces for cell adhesion studies and other microfabrication needs.