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Dynamic force spectroscopy: Analysis of reversible bond-breaking dynamics.

Gregor Diezemann¹, Andreas Janshoff

¹Institut fur Physikalische Chemie, Universitat Mainz, Welderweg 11, 55099 Mainz, Germany. diezeman@uni-mainz.de

The Journal of Chemical Physics

|December 3, 2008

Summary

This study analyzes bond dissociation dynamics under force, revealing how loading rates and rebinding influence rupture forces. Equilibrium constants can be extracted from rupture force measurements, offering insights into molecular interactions.

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Area of Science:

Biophysics
Chemical Physics

Background:

Understanding bond dissociation is crucial for molecular mechanics.
Diffusive processes and external forces significantly impact bond stability.

Purpose of the Study:

To investigate diffusive bond dissociation in a double well potential under external force.
To analyze the influence of finite rebinding probabilities on the dynamic force spectrum.
To determine how parameters like loading rate and linker compliance affect rupture forces.

Main Methods:

Theoretical modeling of barrier crossing dynamics.
Computation of rupture force probability distributions.
Analysis of force-dependent bond dynamics under varying external conditions.

Main Results:

Rupture and rejoining forces depend on loading rates, consistent with potential shape.
At low loading rates, pull and relax modes converge towards equilibrium.
Soft linker compliance can either not affect or predictably alter equilibrium rupture force.

Conclusions:

Finite rebinding probabilities and loading rates critically influence dynamic force spectra.
Equilibrium constants can be determined from equilibrium rupture force measurements.
The study provides a framework for understanding and quantifying molecular bond behavior under force.