Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genetic analysis of a lactococcal plasmid replicon.

F F Xu1, L E Pearce, P L Yu

  • 1Department of Biotechnology, Massey University, Palmerston North, New Zealand.

Molecular & General Genetics : MGG
|May 1, 1991
PubMed
Summary

Researchers sequenced the Lactococcus lactis plasmid pFX2, identifying key replication genes (repA, repB) and a staphylococcal sequence. This analysis aids in constructing novel lactococcal vectors for genetic engineering applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mass Probe of Tetrahedral Symmetry in Atomic Nuclei.

Physical review letters·2026
Same author

[Metastatic leiomyosarcoma of the tonsil: a case report].

Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery·2024
Same author

Emergence of High-Order Deformation in Rotating Transfermium Nuclei: A Microscopic Understanding.

Physical review letters·2024
Same author

[Study on the application value of fecal <i>SDC2</i> gene methylation detection in colorectal cancer screening of urban residents in Zengcheng District in Guangzhou City].

Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine]·2024
Same author

Abnormal Bifurcation of the Double Binding Energy Differences and Proton-Neutron Pairing: Nuclei Close to N=Z Line from Ni to Rb.

Physical review letters·2024
Same author

[A patient with development delay and pallidal and cerebellar lesions caused by MBOAT7 gene variant].

Zhonghua er ke za zhi = Chinese journal of pediatrics·2023

Area of Science:

  • Molecular Biology
  • Microbial Genetics
  • Plasmid Biology

Background:

  • Lactococcus lactis subsp. lactis cryptic plasmids are valuable for developing genetic tools.
  • Understanding plasmid replication mechanisms is crucial for vector construction.
  • pFX2 is a Lactococcus lactis plasmid utilized as a basis for novel vector development.

Purpose of the Study:

  • To determine the complete nucleotide sequence and genetic organization of the pFX2 plasmid.
  • To identify genes and regulatory elements involved in pFX2 replication.
  • To characterize the pFX2 replicon for potential use in lactococcal vector systems.

Main Methods:

  • DNA sequencing of the 2508 bp lactococcal portion of pFX2.
  • Bioinformatic analysis to identify open reading frames and regulatory sequences.
  • Homology searches to compare pFX2 genetic elements with known plasmids.

Main Results:

  • The sequence revealed two replication protein-coding regions, repA and repB, essential for plasmid replication.
  • RepA contains a helix-turn-helix motif, characteristic of DNA-binding proteins.
  • RepB exhibits homology to replication initiation proteins in other Gram-positive bacteria and Mycoplasma.
  • An inverted repeat sequence between repA and repB may function as an attenuator regulating replication.
  • A 215 bp sequence identical to staphylococcal plasmid pE194, including the RSA site, was found upstream of the origin of replication.
  • The overall genetic organization of the pFX2 replicon is similar to pE194 group plasmids.

Conclusions:

  • The genetic makeup of the pFX2 plasmid has been elucidated, revealing key replication determinants.
  • The identified features suggest pFX2 is a valuable platform for developing robust Lactococcus lactis vectors.
  • The similarity to pE194 group plasmids provides insights into conserved replication strategies among Gram-positive bacteria.

Related Experiment Videos