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Updated: Jun 27, 2026

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
Published on: September 19, 2017
The trigger sequence in the GCN4 leucine zipper: alpha-helical propensity and multistate dynamics of folding and
Prem P Chapagain1, Yanxin Liu, Bernard S Gerstman
1Department of Physics, Florida International University, University Park, Miami, Florida 33199, USA. chapagap@fiu.edu
Abstract:
We investigate the importance of the trigger sequence in the folding and dimerization of the GCN4 leucine zipper. We examine the role of the enhanced propensity of the amino acids in the trigger sequence to form an alpha-helix. Using computer simulations, we calculate heat capacities, free energy profiles, and the probability for successful dimerization as a function of the strength of the alpha-helical propensity of the trigger sequence. Our results elucidate the experimentally observed importance of the trigger sequence for dimerization and why it is not necessary for the trigger to have a specific "consensus" sequence of amino acids. We also find that a stronger trigger sequence not only increases the probability for dimerization but also changes the dimerization dynamics by introducing multiple intermediate states.
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