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Updated: Jun 27, 2026

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer
07:25

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

Crosstalk between the androgen receptor and beta-catenin in castrate-resistant prostate cancer

Gang Wang1, Jun Wang, Marianne D Sadar

  • 1Genome Sciences Centre, British Columbia Cancer Agency, Vancouver, British Columbia, Canada.

Cancer Research
|December 3, 2008
PubMed

Insights

Prostate cancer progression to castrate-resistant stages involves androgen receptor (AR) and Wnt/beta-catenin pathway activation. This study shows in vivo evidence of AR and beta-catenin interaction in advanced prostate cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Androgen signaling pathway is crucial in prostate cancer (PC) development and progression.
  • Castrate-resistant prostate cancer (CRPC) is a major challenge in PC treatment.
  • Wnt pathway and beta-catenin are implicated in prostate biology and pathology.

Purpose of the Study:

  • To investigate the genomic similarity of a prostate cancer hollow fiber model to clinical samples.
  • To identify differentially expressed genes during hormonal progression of prostate cancer.
  • To elucidate the role of Wnt/beta-catenin signaling in CRPC progression.

Main Methods:

  • Affymetrix GeneChip analysis for gene expression profiling.
  • Utilized LNCaP hollow fiber model and xenografts from castrated and noncastrated mice.
  • Immunohistochemistry and Western blotting to assess protein expression and localization.

Main Results:

  • Genomic similarity confirmed between the hollow fiber model and clinical samples.
  • Identified differential gene expression during hormonal progression, supporting AR reactivation in CRPC.
  • Demonstrated activation of the Wnt pathway and increased expression of AR and beta-catenin in CRPC.
  • Observed nuclear colocalization and interaction of AR and beta-catenin in CRPC from castrated mice.

Conclusions:

  • The study provides genomic evidence for AR signaling reactivation in CRPC.
  • Aberrant activation of the androgen receptor (AR) via the Wnt/beta-catenin pathway is implicated in CRPC progression.
  • This interaction is specific to the castrate-resistant state in vivo.

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