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Updated: Aug 10, 2026
07:23
Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
Summary
Owl monkeys inoculated with Epstein-Barr (EB) virus developed a malignant lymphoma. A cell line from the diseased monkey confirmed the presence of EB virus, suggesting its potential oncogenic capacity in vivo.
Area of Science:
- Virology
- Oncology
- Primate Models
Background:
- Epstein-Barr virus (EBV) is a human herpesvirus linked to various cancers.
- The oncogenic potential of EBV in non-human primates remains incompletely understood.
Purpose of the Study:
- To investigate the effects of Epstein-Barr virus inoculation in owl monkeys (Aotus trivirgatus).
- To determine if EBV can induce malignant lymphoma and establish an infected cell line in vivo.
Main Methods:
- Owl monkeys were inoculated with EB virus or control material.
- Diseased animals were examined for pathological changes and lymph node abnormalities.
- A cell line was established and characterized using electron microscopy, immunofluorescence, and nucleic acid hybridization.
Main Results:
- One of three inoculated monkeys died from a reticuloproliferative disease resembling malignant lymphoma.
- A herpesvirus identified as EB virus was successfully isolated and characterized from the diseased monkey's lymph node cell line.
- Only the diseased animal showed an antibody response to EB virus; heterophile antibodies were not detected.
Conclusions:
- Owl monkeys can develop malignant lymphoma following EB virus inoculation.
- The study provides evidence supporting the oncogenic capacity of EB virus in vivo.
- The established cell line serves as a valuable tool for further EBV research.
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