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Updated: Jun 27, 2026

A Cell-to-cell Macromolecular Transport Assay in Planta Utilizing Biolistic Bombardment
Published on: August 27, 2010
Probing (macro)molecular transport through cell walls
Giona Kilcher1, Daniela Delneri, Craig Duckham
1School of Pharmacy and Pharmaceutical Sciences, University of Manchester, Oxford Road, Manchester, United Kingdom M13 9PT.
Abstract:
We here report a study on the passive permeability of hydrophobic probes through the cell wall of Saccharomyces cerevisiae. In this study we have prepared a series of fluorescent probes with similar chemical composition and molecular weight ranging from a few hundreds to a few thousands of g mol(-1). Their permeation into the cell body exhibits a clear MW cut-off and the underlying mechanism is governed by the permeation of individual molecules rather than aggregates. We also show that it is possible to reversibly alter the cell wall permeation properties without compromising the essence of its structure, by modifying the polarity/dielectric constant of the wall through solvent exchange.
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