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Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
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Incorporating stimulus-responsive character into filamentous virus assemblies.

Harry Bermudez1, Adam P Hathorne

  • 1Department of Polymer Science & Engineering, University of Massachusetts. Amherst, MA 01003, USA. bermudez@polysci.umass.edu

Faraday Discussions
|December 4, 2008
PubMed
Summary

Researchers modified filamentous bacteriophage particles with elastin-like polypeptide (ELP) motifs to introduce stimulus-responsive behavior. While ELP-phage particles showed reduced infectivity and altered hydrodynamic size, they did not aggregate, indicating potential for controlled self-assembly.

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Area of Science:

  • Biomaterials Science
  • Materials Science
  • Molecular Biology

Background:

  • Controlling interactions in self-assemblies is crucial for functional materials.
  • Filamentous bacteriophage serves as a model system for studying self-assembly.
  • Stimulus-responsive behavior can be introduced by altering surface features.

Purpose of the Study:

  • To introduce stimulus-responsive behavior into filamentous bacteriophage particles.
  • To investigate the effects of elastin-like polypeptide (ELP) motifs on phage particle characteristics.
  • To understand how surface modification impacts self-assembly and material properties.

Main Methods:

  • Recombinant DNA methods to introduce ELP motifs into major coat capsid proteins.
  • Microbiological assays to assess bacterial growth and viral infectivity.
  • Zeta potential, dynamic light scattering (DLS), and calorimetry for particle characterization.

Main Results:

  • Bacteria producing ELP-phage particles exhibited slower growth.
  • ELP-modified phages showed a significant reduction in viral infectivity.
  • ELP-phage particles did not aggregate, but hydrodynamic size varied with ELP motif details.
  • Calorimetry suggested subtle thermal transitions of ELP motifs without macroscopic aggregation.

Conclusions:

  • ELP modification of filamentous bacteriophage is a viable strategy for introducing stimulus-responsive properties.
  • The modifications influence phage infectivity and particle size without inducing aggregation.
  • These findings advance the understanding of self-assembly control for potential applications in functional materials.