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Transferable capacity for gastrointestinal colonization in Enterococcus faecium in a mouse model.

Louis B Rice1, Viera Lakticová, Lenore L Carias

  • 1Medical and Research Services, Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA. louis.rice@va.gov

The Journal of Infectious Diseases
|December 4, 2008
PubMed
Summary

Enterococcus faecium colonization of the gut can lead to infection. Researchers found that E. faecium can transfer gut colonization ability to other strains, independent of antibiotic resistance.

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Genetics

Background:

  • High gastrointestinal colonization by Enterococcus faecium often precedes invasive infections.
  • Factors promoting E. faecium gut colonization, beyond antimicrobial resistance, remain largely unidentified.

Purpose of the Study:

  • To investigate the transferability of gastrointestinal colonization ability among E. faecium strains.
  • To identify potential transmissible factors contributing to E. faecium gut colonization.

Main Methods:

  • Conjugation experiments were performed using a colonization-proficient E. faecium isolate (C68) and non-colonizing recipient strains (D344SRF and GE-1).
  • Transconjugant strains were assessed for their ability to colonize the mouse gastrointestinal tract under selective antibiotic pressure (clindamycin or vancomycin).

Main Results:

  • Transconjugants from both recipient strains (D344SRF and GE-1) demonstrated significantly higher gastrointestinal colonization in mice compared to control strains.
  • This enhanced colonization occurred under selective pressure from clindamycin or vancomycin, indicating a non-resistance-associated mechanism.

Conclusions:

  • Enterococcus faecium clinical isolates possess transmissible factors that promote gastrointestinal colonization.
  • These factors are distinct from antimicrobial resistance mechanisms and play a crucial role in E. faecium gut colonization.