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Related Concept Videos

Drug Distribution: Plasma Protein Binding01:29

Drug Distribution: Plasma Protein Binding

Drugs predominantly attach to plasma proteins, with only a small percentage remaining unbound. The unbound portion can be calculated as one minus the bound fraction. Acidic drugs form large, inactive complexes by reversibly binding to plasma albumin, which prevents them from diffusing across biological barriers. These drug-protein complexes act as reservoirs for the drugs. As the concentration of unbound drugs decreases, these complexes quickly dissociate to release the free drug, maintaining...
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Renal function tests are crucial for assessing kidney health, monitoring disease progression, and evaluating the kidneys' efficiency in waste elimination, fluid balance, and electrolyte regulation. These tests offer critical insights into kidney function, even though routine measurements may appear normal until there is a significant decline in the glomerular filtration rate or GFR. Typically, signs of kidney impairment only become evident when the GFR falls to about 50% of its normal level.
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Related Experiment Video

Updated: Jun 27, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Proteome patterns in uremic plasma.

Uzma Naseeb1, Jawed Shafqat, Theres Jägerbrink

  • 1Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.

Blood Purification
|December 5, 2008
PubMed
Summary

Researchers identified 29 altered proteins in chronic kidney disease (CKD) patients, with 25 proteins found at higher concentrations. This study enhances understanding of CKD proteome changes beyond known markers.

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Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
10:31

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis
14:48

Proteomic Profile of EPS-Urine through FASP Digestion and Data-Independent Analysis

Published on: May 8, 2021

Area of Science:

  • Biochemistry
  • Proteomics
  • Nephrology

Background:

  • Chronic kidney disease (CKD) is associated with altered circulating peptide and protein concentrations.
  • Previous knowledge of CKD-related proteomic changes is incomplete.

Purpose of the Study:

  • To characterize and identify novel protein alterations in plasma from CKD stage 5 patients.
  • To compare the proteome of CKD patients with healthy controls.

Main Methods:

  • Plasma samples from 7 CKD stage 5 patients (pre-dialysis, no comorbidities) and 7 healthy controls were analyzed.
  • Immunodepletion of the top 6 abundant plasma proteins was performed.
  • High-performance liquid chromatography (HPLC) and SDS-PAGE were used, followed by peptide mass fingerprinting (MALDI-MS) and tandem mass spectrometry (ESI-MS/MS) for protein identification.

Main Results:

  • Significant differences in HPLC and SDS-PAGE patterns were observed between CKD and healthy groups.
  • 29 proteins were identified with altered concentrations across 19 HPLC fractions.
  • 25 proteins were found at higher concentrations and 4 at lower concentrations in CKD patients compared to controls.

Conclusions:

  • This study identified 29 differentially abundant proteins in CKD stage 5 plasma.
  • At least 6 new protein components were added to the known altered proteome in CKD.
  • These findings expand the understanding of the CKD proteome and may offer new diagnostic or therapeutic targets.