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Updated: Jun 27, 2026
Doxycycline Loaded Collagen-Chitosan Composite Scaffold for the Accelerated Healing of Diabetic Wounds
Published on: August 21, 2021
Short term effects of doxycycline on matrix metalloproteinases 2 and 9
Nicola Fiotti1, Nicola Altamura, Michèle Moretti
1S C Clinica Medica Generale e Terapia Medica, Dipartimento di Scienze Cliniche, Morfologiche e Tecnologiche, Università degli Studi di Trieste, Trieste, Italy. fiotti@units.it
Purpose:
To investigate the short term effects of Doxycycline on MMP-2 and MMP-9.
Methods:
Short term effects of Doxycycline (100 mg B.I.D.) on plasma levels of MMP-2 and MMP-9 were investigated in 20 healthy subjects; the effects of Doxy, Acetylsalicylic acid, Nitrates, and Enalapril on MMP-9 release from were assessed in isolated polymorphonuclear cells.
Results:
In plasma, MMP-9 activity was reduced (-22%, 95% CI -32/-11; P = 0.002) starting at 12 h after doxy; in vitro, MMP-9 released from stimulated neutrophils was reduced by Doxy (-28%, 95% CI -43/-14; P = 0.001), inhibiting degranulation, and by nitrates (-52%, 95% CI -76/-28 P = 0.005), increasing three times both pro- and active-MMP-9 bound to neutrophils (P = 0.007 and 0.040, respectively).
Conclusions:
Doxy decreases MMP-9 plasma levels by around 20%, within the first 12 h. The mechanism leading to such reduction seems due to the inhibition of PMN degranulation.
