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Complementary Use of Microscopic Techniques and Fluorescence Reading in Studying Cryptococcus-Amoeba Interactions
Published on: June 22, 2019
Atypical micromorphology and uncommon location of cryptococcosis: a histopathologic study using special histochemical
Alexandra Flávia Gazzoni1, Cecília Bittencourt Severo, Marines Bizarro Barra
1Post-graduation Program in Pulmonary Sciences, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. aleflavia@yahoo.com.br
Abstract:
Here we report an unusual case of disseminated cryptococcosis in a patient with AIDS. Although typical Cryptococcus neoformans micromorphology was observed in tongue biopsy, cervical lymph node examination revealed atypical histopathologic findings. These included pseudohyphae, chains of budding yeasts and structures resembling germ tubes. Cryptococcus neoformans infection in supraclavicular lymph nodes was also confirmed by culture. The importance of using special histochemical techniques-Mayer's mucicarmine stain for mucicarminophilic capsule and Grocott's silver stain-in the diagnosis of cryptococcosis is reinforced.
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