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Updated: Jun 27, 2026
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Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
New applications and developments in the use of multiplex ligation-dependent probe amplification
Piotr Kozlowski1, Anna J Jasinska, David J Kwiatkowski
1Laboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland. kozlowp@rose.man.poznan.pl
Electrophoresis
|December 5, 2008
Summary
Multiplex ligation-dependent probe amplification (MLPA) is a versatile DNA copy number analysis tool. Recent enhancements enable new applications like methylation analysis and expression profiling, expanding its utility in genetic research.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- Multiplex ligation-dependent probe amplification (MLPA) is a standard method for quantifying DNA sequence dosage.
- MLPA has been instrumental in identifying numerous disease-causing genomic mutations in genes like DMD, BRCA1, NF1, and TSC2.
Purpose of the Study:
- To review the evolution and expanded applications of MLPA.
- To highlight recent advancements and future potential of MLPA technology.
Main Methods:
- The study reviews modifications to the original MLPA technique.
- Key advancements include chemically synthesized probes for novel probe set creation.
Main Results:
- MLPA has been successfully adapted for methylation status determination, copy number analysis in complex genomic regions, expression profiling, and transgene genotyping.
- Chemically synthesized probes facilitate the development of custom MLPA assays for diverse research needs.
Conclusions:
- MLPA is a highly adaptable technique with a growing range of applications beyond its original scope.
- Future improvements, such as array-MLPA, promise increased throughput and reduced costs for genome-wide association studies.
