Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Liquid-infused silicone catheters reduce fungal burden and inflammation in <i>Candidozyma auris</i> bladder infections.

mSphere·2026
Same author

Liquid-Infused Silicone Catheters Reduce Fungal Burden and Inflammation in <i>Candidozyma auris</i> Bladder Infections.

bioRxiv : the preprint server for biology·2026
Same author

Growing Sustainable Barrier Coatings from Edible Fungal Mycelia.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Paclitaxel neurotoxicity is triggered by epidermal EG5-dependent microtubule fasciculation and X-ROS formation.

Research square·2025
Same author

The Orientation of Human Fibrinogen at Biomedically Relevant Polydimethylsiloxane-Water Interfaces.

Langmuir : the ACS journal of surfaces and colloids·2025
Same author

Multi-component liquid-infused systems: a new approach to functional coatings.

Industrial chemistry & materials·2024

Related Experiment Video

Updated: Jun 27, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

Probing the extracellular matrix with sum-frequency-generation spectroscopy.

Caitlin Howell1, Mark-Oliver Diesner, Michael Grunze

  • 1Applied Physical Chemistry and Biophysical Chemistry, University Heidelberg, Im Neuenheimer Feld 263, 69120 Heidelberg, Germany.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 5, 2008
PubMed
Summary

Vibrational sum-frequency-generation (SFG) spectroscopy can characterize extracellular matrix (ECM) coatings on gold surfaces, even with adhered cells. This technique is robust, showing minimal cell damage and consistent fibronectin signals.

More Related Videos

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy
09:43

Interfacial Molecular-level Structures of Polymers and Biomacromolecules Revealed via Sum Frequency Generation Vibrational Spectroscopy

Published on: August 13, 2019

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy
07:19

Preparation of Extracellular Matrix Protein Fibers for Brillouin Spectroscopy

Published on: September 15, 2016

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix
09:40

A Rapid, Scalable Method for the Isolation, Functional Study, and Analysis of Cell-derived Extracellular Matrix

Published on: January 4, 2017

Area of Science:

  • Surface Science
  • Spectroscopy
  • Biomaterials

Background:

  • Characterizing the extracellular matrix (ECM) is crucial for understanding cell-material interactions.
  • Vibrational spectroscopy techniques are valuable for molecular-level surface analysis.
  • Distinguishing between surface coatings and adherent cells in spectroscopic analysis presents challenges.

Purpose of the Study:

  • To investigate the utility of vibrational sum-frequency-generation (SFG) spectroscopy for analyzing fibronectin coatings on gold surfaces.
  • To determine if SFG spectroscopy can differentiate between fibronectin coatings and adherent embryonic fibroblasts.
  • To assess the feasibility of using SFG spectroscopy for in vitro ECM characterization.

Main Methods:

  • Fibronectin-coated gold surfaces, with and without adherent embryonic fibroblasts, were analyzed using SFG spectroscopy.
  • SFG spectra were compared with infrared reflection-absorption spectroscopy (IRRAS) data.
  • Experiments included analysis of cells on bare gold and assessment of laser-induced cell damage.

Main Results:

  • SFG spectra showed minimal differences between fibronectin-coated surfaces with and without cells, suggesting cells do not significantly contribute to the SFG signal.
  • IRRAS spectra exhibited noticeable differences, highlighting SFG's specificity for the fibronectin layer.
  • SFG spectra of cells on fibronectin-coated gold were similar to those on bare gold, and no significant laser-induced cell damage was observed.

Conclusions:

  • SFG spectroscopy is a feasible tool for characterizing the ECM layer adjacent to a gold substrate beneath cells.
  • The technique can effectively probe the fibronectin coating independently of adherent cell layers.
  • SFG spectroscopy holds potential for in vitro examination of the ECM.