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Updated: Jun 27, 2026
A Mouse Model for Corneal Neovascularization by Alkali Burn
Published on: June 30, 2023
Delayed neovascularization in inflammation-induced corneal neovascularization in interleukin-10-deficient mice
Branka Samolov1, Anders Kvanta, Ingeborg van der Ploeg
1Department of Clinical Neuroscience, Section for Ophthalmology and Vision, St Erik's Eye Hospital, Karolinska Institute, Stockholm, Sweden. branka.samolov@sankterik.se
Purpose:
To investigate the potential modulatory role of interleukin-10 (IL-10) in the suture model for corneal neovascularization.
Methods:
Neovascularized areas were measured on corneal flat-mounts in IL-10(-/-) and wild-type C57BL6 mice. The inflammatory cellular response was characterized with immunohistochemistry. Gene expression was measured by real-time polymerase chain reaction.
Results:
IL-10(-/-) mice showed a delayed neovascular response compared to wild-type animals at day 6 after suture, when approximately half of the cornea was neovascularized. No apparent differences in inflammatory responses or in messenger RNA (mRNA) expression for proangiogenic factors were detected in IL-10(-/-) versus wild-type mice.
Conclusion:
IL-10 appears to have a proangiogenic effect in the suture model for corneal neovascularization that cannot be explained by either IL-10's anti-inflammatory effect or apparent cross-talk with the angiogenic factors vascular endothelial growth factor (VEGF)-A, metalloproteinase (MMP)-2 and MMP-9, angiopoietin (Ang)-1 and Ang-2.
