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Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Chromatography: Introduction01:10

Chromatography: Introduction

Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
The phase in which the compounds linger or on which the compounds adsorb is called the stationary phase, whereas the mobile phase is the solvent that carries the solutes to be analyzed. In traditional column chromatography, the mixture flows through the stationary phase, and the compounds partition between the stationary and mobile phases...
Chromatographic Methods: Terminology01:18

Chromatographic Methods: Terminology

Chromatography is an analytical technique widely used in fields such as chemistry, biology, environmental science, and pharmaceuticals to separate the components of a mixture and identify substances between them. The process of chromatography is based on the interactions between two distinct phases: the stationary phase and the mobile phase. The stationary phase is fixed in place by a supporting material, while the mobile phase moves over it, carrying the solutes. As the mobile phase travels,...
Optimizing Chromatographic Separations01:15

Optimizing Chromatographic Separations

Optimizing chromatographic separations is crucial for obtaining clean separations in a minimum amount of time. Optimization is required for several factors, including kinetic effects related to band broadening, plate height, capacity factor, and separation factor.
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Principles Of Column Chromatography01:13

Principles Of Column Chromatography

The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
Chromatographic Methods: Classification01:12

Chromatographic Methods: Classification

Chromatographic techniques are classified in three ways: the classification is based on the physical state of the stationary and mobile phases, how the mobile phase and the stationary phase contact each other, or through the chemical or physical processes that isolate the components of the sample. Typically, the mobile phase is either a liquid or gas, while the stationary phase is either a solid or a liquid layer applied to a solid surface.
Chromatographic techniques are typically named by...

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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography
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Chromatographic Fingerprinting by Template Matching for Data Collected by Comprehensive Two-Dimensional Gas Chromatography

Published on: September 2, 2020

Data analysis programs for comprehensive two-dimensional chromatography.

Minna Kallio1, Maarit Kivilompolo, Sami Varjo

  • 1Laboratory of Analytical Chemistry, Department of Chemistry, University of Helsinki, P.O. Box 55, FI-00014 Helsinki, Finland.

Journal of Chromatography. A
|December 5, 2008
PubMed
Summary
This summary is machine-generated.

New laboratory software offers user-friendly visualization and interpretation for comprehensive two-dimensional chromatography (LC x LC and GC x GC) data. These versatile programs enable detailed analysis and comparison of complex chromatographic datasets without instrument restrictions.

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Area of Science:

  • Analytical Chemistry
  • Chromatography
  • Data Visualization

Background:

  • Comprehensive two-dimensional chromatography (LC x LC, GC x GC) generates complex datasets.
  • Existing software may be instrument-specific or lack user-friendly interfaces for data interpretation.

Purpose of the Study:

  • To develop user-friendly, instrument-independent software for analyzing LC x LC and GC x GC data.
  • To facilitate the visualization and interpretation of complex chromatographic results.

Main Methods:

  • Development of laboratory-written programs for data processing.
  • Implementation of visualization tools for 2D and 3D plots.
  • Features for comparing multiple datasets and extracting peak information.

Main Results:

  • Successfully created versatile software for comprehensive two-dimensional chromatography data analysis.
  • Enabled visualization of 2D and 3D chromatographic plots.
  • Provided capabilities for direct comparison of chromatograms and determination of peak parameters.

Conclusions:

  • The developed programs offer an accessible solution for interpreting complex LC x LC and GC x GC data.
  • Instrument independence and user-friendly features enhance the utility of the software in diverse laboratory settings.