Related Experiment Video
Updated: Jun 27, 2026
Anterior Cruciate Ligament Transection and Synovial Fluid Lavage in a Rodent Model to Study Joint Inflammation and Posttraumatic Osteoarthritis
Published on: September 2, 2025
Magnetic resonance T(1 rho) imaging of osteoarthritis: a rabbit ACL transection model
Jesus Lozano1, Ehsan Saadat, Xiaojuan Li
1University of California, San Francisco School of Medicine, San Francisco, CA 94158, USA.
Objective:
The objective of this study was to develop quantitative T(1 rho)-weighted magnetic resonance imaging methodology for the detection and characterization of cartilage degeneration in a rabbit anterior cruciate ligament (ACL) transection model.
Methods:
The right knee ACLs of 18 adult female New Zealand white rabbits were transected. The left knee joint served as a sham control. The rabbits were euthanized at 3 (Group 1), 6 (Group 2) and 12 (Group 3) weeks postoperatively. High-resolution 3D fat-saturated spoiled gradient echo images and T(1 rho)-weighted images were obtained in both the sagittal and axial planes at 3 T using a quadrature wrist coil. Following MR analysis, histological slides from the lateral femoral condyle cartilage were graded using the Mankin grading system.
Results:
For all three groups, the average overall T(1 rho) values were significantly higher in the ACL-transected knee compared to control knee, and the percentage differences in T(1 rho) values between ACL-transected and control increased with the duration of time after transection. The average Mankin score for ACL-transected knees was higher than that for control for each time point, but this difference was statistically significant only for all groups combined.
Conclusions:
This study demonstrates the feasibility of using T(1 rho)-weighted imaging as a useful tool in the detection and quantification of cartilage damage in all knee compartments in an ACL-transected rabbit model of cartilage degeneration.
