Related Experiment Videos
Reactivation of metal-requiring apoenzymes by phytochelatin-metal complexes
J Thumann1, E Grill, E L Winnacker
1Lehrstuhl für Pharmazeutische Biologie, Universität München, Germany.
FEBS Letters
|June 17, 1991
Abstract:
The enzymatically inactive, metal-requiring apoforms of diamino oxidase and of carbonic anhydrase were reactivated by copper-and zinc-phytochelatin complexes, respectively. The level and the rate of reactivation effected by metal complexes consisting of poly(gamma-glutamylcysteinyl)glycine as well as by the respective free metal ion were compared. An efficient transfer of zinc and copper from phytochelatin-complexes to apoenzymes was observed in vitro.