Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

Sulfur Assimilation

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The structure of NAD<sup>+</sup> consuming protein Acinetobacter baumannii TIR domain shows unique kinetics and conformations.

The Journal of biological chemistry·2023

Same author

Emergence of allostery through reorganization of protein residue network architecture.

The Journal of chemical physics·2023

Same author

Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation.

Nature communications·2020

Same author

Structure of the cell-binding component of the <i>Clostridium difficile</i> binary toxin reveals a di-heptamer macromolecular assembly.

Proceedings of the National Academy of Sciences of the United States of America·2020

Same author

Tuning Allostery through Integration of Disorder to Order with a Residue Network.

Biochemistry·2020

Same author

Specificity and selectivity in post-translational biotin addition.

Biochemical Society transactions·2018

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

Biotin sensing at the molecular level.

Dorothy Beckett¹

¹Department of Chemistry and Biochemistry, College of Chemical and Life Sciences, University of Maryland, College Park, MD 20742, USA. dbeckett@umd.edu

The Journal of Nutrition

|December 6, 2008

Summary

This summary is machine-generated.

Biotin sensing in humans involves the enzyme biotin holoenzyme ligase (holocarboxylase synthetase), which regulates gene expression. Its function is more complex than the bacterial mechanism, involving both metabolic and transcriptional roles.

More Related Videos

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Related Experiment Videos

Last Updated: Jun 27, 2026

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia

Published on: September 12, 2025

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

Area of Science:

Biochemistry
Molecular Biology
Gene Regulation

Background:

Biotin is essential for transcription across species.
Biotin protein ligase transmits biotin demand to gene expression.
Bacterial biotin sensing involves kinetically controlled protein interactions.

Purpose of the Study:

To elucidate the molecular mechanism of biotin sensing at the transcriptional level in humans.
To characterize the role of human biotin holoenzyme ligase (holocarboxylase synthetase) in gene expression.

Main Methods:

Biochemical assays to measure enzyme kinetics.
Analysis of protein:protein interactions.
Investigation of biotinylation of carboxylases and histones.

Main Results:

Human biotin holoenzyme ligase (holocarboxylase synthetase) has dual roles in metabolism and transcription.
The control of human holocarboxylase synthetase is significantly more complex than bacterial systems.
Protein:protein interaction competition is a key regulatory mechanism.

Conclusions:

Human biotin sensing at the transcriptional level is complex and not fully understood.
Biotin holoenzyme ligase (holocarboxylase synthetase) is a central regulator of biotin-mediated gene expression.
Further research is needed to fully characterize the human biotinylation regulatory network.