Related Experiment Video

Updated: Jun 27, 2026

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter
12:15

Quantification of three DNA Lesions by Mass Spectrometry and Assessment of Their Levels in Tissues of Mice Exposed to Ambient Fine Particulate Matter

Published on: May 29, 2019

GST M1 polymorphism associates with DNA oxidative damage and mortality among hemodialysis patients

Yi-Sheng Lin1, Szu-Chun Hung, Yau-Huei Wei

  • 1Division of Nephrology, Taipei City Hospital Zhongxiao Branch, Taipei, Taiwan.

Journal of the American Society of Nephrology : JASN
|December 6, 2008
PubMed

Insights

Patients lacking Glutathione S-transferase M1 (GST M1) activity show increased DNA damage and a doubled mortality risk in hemodialysis. This highlights GST M1

Area of Science:

  • Nephrology
  • Genetics
  • Biochemistry

Background:

  • Leukocyte 8-hydroxy-2'-deoxyguanosine (8-OHdG) indicates DNA damage from oxidative stress in maintenance hemodialysis (MHD) patients.
  • Glutathione S-transferase M1 (GST M1) enzymes protect DNA from oxidative damage.

Purpose of the Study:

  • To investigate the association between the Glutathione S-transferase M1 (GST M1) gene polymorphism and 8-OHdG levels.
  • To determine the impact of the GST M1 gene polymorphism on all-cause mortality in MHD patients.

Main Methods:

  • Compared 8-OHdG levels and GST M1 genotype frequencies in 488 MHD patients and 372 healthy controls.
  • Utilized multivariate Cox regression to assess mortality risk associated with the GST M1(-) genotype.

Main Results:

  • The GST M1(-) genotype was linked to significantly higher leukocyte 8-OHdG levels (P < 0.001).
  • Patients with GST M1(-) genotype had approximately double the risk of all-cause mortality (HR 2.24).
  • Elevated 8-OHdG levels were observed in GST M1(-) patients with specific DNA repair gene polymorphisms or those dialyzed with cellulose membranes.

Conclusions:

  • Individuals without GST M1 activity are more susceptible to oxidative stress and face a higher mortality risk.
  • The GST M1(-) genotype is a significant risk factor for mortality in maintenance hemodialysis patients.

More Related Videos

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform06:21

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Published on: May 10, 2024

Related Concept Videos

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu01:29

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu

Genetic variations significantly influence drug response through pharmacokinetics, receptor interactions, and biologic milieu modifications. Pharmacokinetic alterations impact drug metabolism and clearance, affecting efficacy and toxicity. Variants in drug-metabolizing enzymes, such as CYP2C9 and CYP2C19, alter drug activation and elimination. For example, CYP2C9 loss-of-function variants require lower warfarin doses to prevent excessive bleeding, while CYP2C19 variants reduce clopidogrel...
Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase01:11

Pharmacogenetics of Drug Targets: β₂-Adrenergic Receptors, Apo E, Thymidylate Synthase

Genetic polymorphisms in drug targets have emerged as critical determinants of interindividual variability in drug response and toxicity. Pharmacogenomic investigations increasingly focus on identifying these variations to personalize and optimize therapeutic interventions. A drug target may be a receptor, enzyme, or signaling protein involved in pharmacologic responses or disease-related pathways. While early pharmacogenetic studies focused primarily on drug metabolism, current research...
Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase01:27

Pharmacogenetics of Phase II Enzymes: N-acetyltransferase, Thiopurine S-methyltransferase, UDP-glucuronosyltransferase

Phase II biotransformation reactions are essential for detoxifying and eliminating xenobiotics, including many pharmaceutical compounds. These reactions typically involve conjugation, the covalent attachment of polar endogenous groups such as glucuronic acid, sulfate, methyl, or acetyl moieties to functional groups introduced during Phase I metabolism. The resulting conjugates are more water-soluble, enabling efficient renal or biliary excretion.The major classes of Phase II enzymes include...