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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing02:01

Pre-mRNA Processing

In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...

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Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
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ASTD: The Alternative Splicing and Transcript Diversity database.

Gautier Koscielny1, Vincent Le Texier, Chellappa Gopalakrishnan

  • 1European Bioinformatics Institute, European Molecular Biology Laboratory, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK.

Genomics
|December 9, 2008
PubMed
Summary
This summary is machine-generated.

The Alternative Splicing and Transcript Diversity database (ASTD) provides comprehensive data on alternative transcripts, including splicing, transcription initiation, and polyadenylation variants. This resource aids in understanding gene expression complexity and tissue-specific isoforms.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Alternative splicing, transcription initiation, and polyadenylation generate transcript diversity.
  • Existing databases like ASD and ATD have been superseded.
  • Understanding these variations is crucial for comprehending gene regulation.

Purpose of the Study:

  • To introduce the Alternative Splicing and Transcript Diversity database (ASTD).
  • To provide integrated data on multiple types of transcript variation.
  • To facilitate the study of alternative transcripts and their functions.

Main Methods:

  • Mapping transcribed sequences to human, mouse, and rat genomes.
  • Utilizing an extended computational pipeline from previous databases (ASD, ATD).
  • Incorporating data on splicing, transcription initiation, and polyadenylation variants.

Main Results:

  • ASTD integrates vast collections of alternative transcripts.
  • Identified splicing, transcription initiation, and polyadenylation variants in a significant percentage of human genes (68%, 68%, 62%).
  • Experimental validation confirmed a subset of predicted alternative forms.

Conclusions:

  • ASTD offers a comprehensive resource for alternative transcript data.
  • The database supports the identification of tissue-specific isoforms through query tools.
  • ASTD advances the study of transcriptomic complexity and gene expression.