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Updated: Jun 27, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
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"Spot and hop": internal referencing for surface plasmon resonance imaging using a three-dimensional microfluidic

Mark A Eddings1, Josh W Eckman, Carlos A Arana

  • 1Department of Bioengineering, University of Utah, Salt Lake City, UT 84112, USA.

Analytical Biochemistry
|December 9, 2008
PubMed
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A new "spot and hop" referencing technique improves surface plasmon resonance imaging. This method allows for internal referencing in microfluidic networks, enhancing data quality for biosensor applications.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Surface Science

Background:

  • Surface plasmon resonance (SPR) imaging is a label-free optical technique for studying molecular interactions.
  • Current SPR imaging systems often face challenges with instrument drift and nonspecific binding, impacting data accuracy.
  • Parallel processing microfluidic networks offer high-throughput analysis but require robust referencing strategies.

Purpose of the Study:

  • To introduce and validate a novel internal referencing technique,
  • spot and hop
  • for SPR imaging systems.
  • To demonstrate the effectiveness of this technique in improving data quality within a parallel processing microfluidic network.
  • To assess the performance of the technique across various experimental conditions relevant to biosensing.

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Last Updated: Jun 27, 2026

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Main Methods:

  • Development of the "spot and hop" referencing technique for individual flow cell referencing.
  • Integration of the technique into a 48-flow-cell parallel processing microfluidic device coupled with SPR imaging.
  • Performance evaluation through kinetic studies, including "rise and fall" time, ligand preconcentration, immobilization, analyte binding, and regeneration.

Main Results:

  • The "spot and hop" technique successfully enabled internal referencing for each flow cell.
  • Demonstrated significant correction for nonspecific binding and instrument drift.
  • Achieved high data quality across all tested regions of interest and experimental conditions.
  • Validated the performance of a 48-flow-cell device utilizing the novel referencing method.

Conclusions:

  • The "spot and hop" technique provides effective internal referencing for SPR imaging in microfluidic systems.
  • This approach significantly enhances the reliability and quality of data generated by array-based optical biosensors.
  • The integration of parallel processing fluidics with imaging systems, enabled by this technique, broadens the scope and throughput of biosensing applications.