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An NAD-specific glutamate dehydrogenase from cyanobacteria. Identification and properties
1Departamento de Bioquimica Vegetal y Biologia Molecular, Universidad de Sevilla y CSIC, Spain.
FEBS Letters
|July 8, 1991
Abstract:
The unicellular cyanobacterium Synechocystis sp. PCC 6803 presents a hexameric NAD-specific glutamate dehydrogenase with a molecular mass of 295 kDa. The enzyme differs from the NADP-glutamate dehydrogenase found in the same strain and is coded by a different gene. NAD-glutamate dehydrogenase shows a high coenzyme specificity, catalyzes preferentially glutamate formation and presents Km values for ammonium, NADH and 2-oxoglutarate of 4.5 mM, 50 microM and 1.8 mM respectively. An animating role for the enzyme is discussed.