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A functionally split pathway for lysine synthesis in Corynebacterium glutamicium.

B Schrumpf1, A Schwarzer, J Kalinowski

  • 1Institut für Biotechnologie, Jülich, Federal Republic of Germany.

Journal of Bacteriology
|July 1, 1991
PubMed
Summary
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Corynebacterium glutamicum utilizes two pathways for L-lysine synthesis. The dehydrogenase pathway is not essential but is crucial for efficiently processing increased metabolic precursors.

Area of Science:

  • Microbiology
  • Biochemistry
  • Metabolic Engineering

Background:

  • Procaryotes synthesize D,L-diaminopimelate and L-lysine via three known pathways.
  • Escherichia coli, Bacillus subtilis, and Bacillus sphaericus each utilize only one pathway.
  • Corynebacterium glutamicum possesses enzyme activities suggesting dual pathway utilization.

Purpose of the Study:

  • To investigate the functional capacity of both the dehydrogenase and succinylase pathways for L-lysine synthesis in Corynebacterium glutamicum.
  • To determine the essentiality and role of the dehydrogenase pathway in managing metabolic flux.

Main Methods:

  • Enzyme activity assays in wild-type bacteria.
  • Gene-directed mutagenesis to create strains with interrupted ddh genes (inactivating the dehydrogenase pathway).

Related Experiment Videos

  • Analysis of lysine formation and intermediate accumulation in engineered strains.

    • Main Results:

    • Corynebacterium glutamicum can utilize both the dehydrogenase and succinylase pathways for D,L-diaminopimelate and L-lysine synthesis.
    • Mutants lacking the dehydrogenase pathway remain prototrophic, confirming succinylase pathway function.
    • Inactivation of the dehydrogenase pathway in high-flux strains reduced lysine production and caused N-succinyl-diaminopimelate accumulation.

    Conclusions:

    • Both D,L-diaminopimelate/L-lysine synthesis pathways are functional in Corynebacterium glutamicum.
    • The dehydrogenase pathway is dispensable for growth but essential for efficient handling of increased precursor flow.
    • Metabolic engineering strategies in C. glutamicum must consider the role of the dehydrogenase pathway.