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Updated: Jun 27, 2026

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
Published on: April 25, 2025
Spectral heterogeneity of PRODAN fluorescence in isotropic solvents revealed by multivariate photokinetic analysis
Brad A Rowe1, Carol A Roach, Joanna Lin
1Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware 19716, USA.
Abstract:
This paper describes a multivariate analysis of the fluorescence emission of 6-propionyl-2-dimethylaminonaphthalene (PRODAN) in a series of isotropic solvents of differing polarity and hydrogen-bonding ability. Multivariate methods distill the essential features from spectral data matrices so that the structural details that are embedded within the data are revealed to the analyst. In the aprotic solvents investigated, the analysis reveals a pair of emission components that have emission maxima that scale with the orientational polarizability. In the alcohols, short-lived, polarity-independent blue bands tentatively attributed to neutral hydrogen-bonded solute-solvent complexes form and relax prior to emission from paired bands that have Stokes shifts that scale with the solvent hydrogen-bonding ability rather than the polarity. In water, the short-lived blue bands were not observed, but the shift in the paired bands did scale with the solvent hydrogen-bonding ability.
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