Related Experiment Video
Updated: Jun 27, 2026

Identifying Microglia and Peripheral Infiltrating Macrophages in the Injured Spinal Cords Using Flow Cytometry
Published on: June 24, 2025
Inflammatory mediators of cerebrospinal fluid from patients with spinal cord injury
Ming-Cheng Tsai1, Chih-Peng Wei, Dan-Yang Lee
1Department of Neurosurgery, Shin Kong Wu Ho-Su Memorial Hospital, and Graduate Institute of Cell and Molecular Biology, Taipei Medical University, Taipei 111, Taiwan.
Background:
The guidelines of MP treatment for acute SCI are still under debate. We examined the inflammatory mediators of CSF in patients with SCI and assessed the effect of MP treatment.
Methods:
We studied 7 patients with acute SCI at the cervical level and examined the mediators of CSF in patients by cytokine antibody array, ELISA and gelatin zymography.
Results:
We found that levels of IL-6, IL-8, monocyte chemoattractant protein-1, neutrophil-activating peptide 2, intracellular adhesion molecule-1, soluble Fas, tissue inhibitors of metalloproteinase 1, and matrix metalloproteinases-2 and -9 were upregulated in patients with complete SCI without MP treatment as compared to patients with MP treatments, incomplete SCI, or controls. Nerve growth factor was upregulated in patients with MP treatment.
Conclusions:
We suggest that a neuroinflammatory CSF profile after complete SCI could be suppressed with MP treatment via downregulating the expression of various cytokines.
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