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Related Concept Videos

Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
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Peripheral thermosensation is the perception of external temperature. A change in temperature (on the surface of the skin and other tissues) is detected by a family of temperature-sensitive ion channels called Transient Receptor Potential, or TRP, receptors. These receptors are located on free nerve endings. Those detecting cold temperatures are closer to the surface of the skin than the nerve endings detecting warmth. These thermoTRP channels, while temperature selective, have relatively...
G-Protein Gated Ion Channels01:21

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GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
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Mechanically-gated Ion Channels01:12

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Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
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Related Experiment Video

Updated: Jun 27, 2026

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
08:27

Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy

Published on: January 7, 2019

TRPC channels and store-operated Ca(2+) entry.

Ginés M Salido1, Stewart O Sage, Juan A Rosado

  • 1Department of Physiology (Cell Physiology Research Group), University of Extremadura, Cáceres 10071, Spain.

Biochimica Et Biophysica Acta
|December 9, 2008
PubMed
Summary
This summary is machine-generated.

Store-operated calcium entry (SOCE) involves calcium (Ca2+) influx through channels. This review examines the controversial role of TRPC proteins in SOCE mechanisms, contrasting evidence for and against their function as store-operated calcium channels.

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Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels
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Single-Cell Calcium Imaging for Studying the Activation of Calcium Ion Channels

Published on: December 13, 2024

Area of Science:

  • Cellular Physiology
  • Molecular Biology
  • Ion Channel Research

Background:

  • Store-operated calcium entry (SOCE) is a primary pathway for cellular calcium influx.
  • Identifying the specific channels responsible for SOCE has been challenging for two decades.
  • While I(CRAC) is well-characterized, other store-operated currents exist.

Purpose of the Study:

  • To review the evidence for and against TRPC proteins functioning as store-operated calcium channels.
  • To provide an overview of the ongoing debate surrounding TRPC proteins in SOCE.

Main Methods:

  • Literature review of studies investigating TRPC proteins and SOCE.
  • Analysis of experimental data and theoretical arguments.
  • Comparison of different store-operated calcium currents.

Main Results:

  • TRPC proteins have been proposed as candidates for store-operated calcium channels.
  • Significant controversy exists regarding the direct role of TRPC proteins in SOCE.
  • Evidence supporting and refuting TRPC involvement in agonist-activated calcium entry is presented.

Conclusions:

  • The precise identity of TRPC proteins involved in SOCE remains a subject of debate.
  • Further research is needed to definitively establish the role of TRPC proteins in store-operated calcium entry.
  • Understanding SOCE mechanisms is crucial for various physiological processes.