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Updated: Jun 27, 2026

Mouse Round Spermatid Injection
Published on: January 26, 2024
Clinical data and parenthood of 63 infertile and Y-microdeleted men
Catherine Patrat1, Thierry Bienvenu, Laurent Janny
1Assistance Publique - Hôpitaux de Paris, Paris, France. catherine.patrat@cch.aphp.fr
Objective:
To collect follow-up data for infertile men with Y microdeletion.
Design:
Retrospective, observational survey.
Setting:
Multicenter IVF units associated with genetics laboratories.
Patient(S):
Sixty-three patients with Y microdeletion.
Intervention(S):
Karyotype analysis, Y microdeletion screening, and assisted reproductive technology.
Main Outcome Measures:
Medical history, karyotype, nature of the AZF deletion, semen parameters, testis biopsy results, choice of assisted reproductive technology, and results of intracytoplasmic sperm injection (ICSI).
Results:
Abnormal karyotypes were found in 8 men (12.7%), who were azoospermic except 1. Of these 8 men, 5 presented a combined AZFb+c deletion, and 3 had a deletion in AZFc only. Most men (39 of 63) were azoospermic, 3 were cryptoazoospermic, and 19 had extreme oligozoospermia (sperm concentration =1.10(6)/mL). Sperm concentration above 1.10(6)/mL was found for 2 men (3.1%). A testis biopsy was performed in 27 azoospermic men, resulting in positive sperm extraction in 6 cases. To date, 42 ICSI cycles with either testicular (n = 5) or ejaculated spermatozoa (n = 37) have been carried out in 23 couples with male partners with AZFc deletion. Eighteen clinical pregnancies were obtained, leading to the birth of 14 babies. Donor insemination had been chosen by 28 couples, leading to the birth of 9 children.
Conclusion:
Karyotype analysis should be systematically performed in Y microdeleted men. Intracytoplasmic sperm injection can be offered to half of AZFc-deleted patients, providing real opportunities to have a child.
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