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Updated: Jun 27, 2026

Functionalized Spirocyclic Heterocycle Synthesis and Cytotoxicity Assay
Published on: February 9, 2021
Cytotoxic benzil and coumestan derivatives from Tephrosia calophylla
Seru Ganapaty1, Guttula Veera Kantha Srilakshmi, Steve Thomas Pannakal
1Pharmacognosy and Phytochemistry Division, Department of Pharmaceutical, Sciences, Andhra University, Visakhapatnam 530 003, Andhra Pradesh, India. ganapatyseru@gmail.com
Abstract:
A benzil, calophione A, 1-(6'-Hydroxy-1',3'-benzodioxol-5'-yl)-2-(6''-hydroxy-2''-isopropenyl-2'',3''-dihydro-benzofuran-5''-yl)-ethane-1,2-dione and three coumestan derivatives, tephcalostan B, C and D were isolated from the roots of Tephrosia calophylla. Their structures were deduced from spectroscopic data, including 2D NMR (1)H-(1)H COSY and (13)C-(1)H COSY experiments. Compounds were evaluated for cytotoxicity against RAW (mouse macrophage cells) and HT-29 (colon cancer cells) cancer cell lines and antiprotozoal activity against various parasitic protozoa. Calophione A exhibited significant cytotoxicity with IC(50) of 5.00 (RAW) and 2.90microM (HT-29), respectively.
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