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[Immunoturbidimetric determination of C-3 components of complement systems using nonlinear calibration]
J Rohácek1, V Semrád, E Klierová
1Abteilung der Klinischen Biochemie, CSFR.
Zeitschrift Fur Medizinische Laboratoriumsdiagnostik
|January 1, 1991
Abstract:
A method for the immunoturbidimetric analysis of the C-3-component in the complement system was elaborated by means of the antiserum Q-SwAHu/C3 USOL (SEVAG) Praha. A diluted human control serum USOL (SEVAG) Praha with declared values of plasma proteins was applied as a standard solution. The relation between concentration and absorption in an eight step calibration series is well described by a parabola of the 2nd degree. The precision in series and the accuracy of the method are mentioned. The proposed technique is in a relatively good correlation with the radial immunodiffusion according to MANCINI.