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Imipenem resistance in pseudomonas aeruginosa PAO: mapping of the OprD2 gene
J P Quinn1, A Darzins, D Miyashiro
1Department of Medicine, Michael Reese Hospital, Chicago, Illinois 60616.
Antimicrobial Agents and Chemotherapy
|April 1, 1991
Abstract:
Carbapenem antibiotics have been shown to penetrate the outer membrane of Pseudomonas aeruginosa through a unique porin protein, OprD2. We mapped the OprD2 gene by conjugation using plasmid FP2 and by transduction using phage F116L. This gene maps between 71 and 75 min on the PAO1 chromosome.
Insights
Carbapenem antibiotics enter Pseudomonas aeruginosa via the OprD2 porin. Researchers mapped the OprD2 gene
Area of Science:
- Microbiology
- Genetics
- Pharmacology
Background:
- Pseudomonas aeruginosa is an opportunistic pathogen.
- Carbapenem antibiotics are crucial for treating infections.
- Outer membrane proteins like OprD2 are key for antibiotic entry.
Purpose of the Study:
- To map the genetic location of the OprD2 gene in Pseudomonas aeruginosa.
- To understand the genetic basis of carbapenem antibiotic penetration.
Main Methods:
- Gene mapping using bacterial conjugation with plasmid FP2.
- Gene mapping using bacteriophage transduction with phage F116L.
Main Results:
- The OprD2 gene was successfully mapped on the PAO1 chromosome.
- The gene's location was precisely determined to be between 71 and 75 minutes.
Conclusions:
- The genetic locus of OprD2 was identified.
- This mapping provides a foundation for further studies on OprD2 function and regulation.