Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Bionanotubule formation from surface-attached liposomes using electric fields.

Josemar A Castillo1, Daniel M Narciso, Mark A Hayes

  • 1Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, USA.

Langmuir : the ACS Journal of Surfaces and Colloids
|December 10, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Emerging capabilities in microscale separations and bioanalysis.

Analytical and bioanalytical chemistry·2026
Same author

Quantifying and Minimizing the Variance of Gradient Insulator-Based Dielectrophoresis.

Micromachines·2026
Same author

Dielectrophoresis reveals stimulus-induced remodeling of insulin granule subpopulations.

Biophysical journal·2026
Same author

Dielectrophoresis Reveals Stimulus-Induced Remodeling of Insulin Granule Subpopulations.

bioRxiv : the preprint server for biology·2025
Same author

Streaming-Particle Method for Dielectrophoretic Characterization.

Electrophoresis·2025
Same author

Gradient Insulator-Based Dielectrophoresis of Gold Nanoparticles.

Electrophoresis·2025
Same journal

Metal-Organic Framework Multizyme Colloids with Joint Antioxidant and Protease Function.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Morphology Engineering of Co<sub>3</sub>O<sub>4</sub> via Cetyltrimethylammonium Bromide-Mediated ZIF-67 Synthesis for Efficient Photo-Assisted Electrooxidation of Methanol.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Speciation of Silanol Groups on Commercial Precipitated Silicas via IR Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Hydrogen Plasma-Driven Surface Defect Engineering of ZnO Nanorods: Correlating Electronic Structure and Photoelectrochemical Performance.

Langmuir : the ACS journal of surfaces and colloids·2026
Same journal

Cooperative Self-Assembly of Nanoparticle-Encapsulating Hybrid Protein Cages.

Langmuir : the ACS journal of surfaces and colloids·2026
See all related articles

Electric fields enable liposome nanotubule formation. Modest voltages create stable, aligned hollow cylinders, with charged lipids decreasing voltage requirements and cholesterol hindering growth.

Area of Science:

  • Biophysics
  • Materials Science
  • Nanotechnology

Background:

  • Liposomes are versatile vesicles with applications in drug delivery and biomimetic systems.
  • Fabricating complex liposomal structures like nanotubules is challenging.

Purpose of the Study:

  • To investigate the spontaneous formation of membrane-bound nanotubules from liposomes using electric fields.
  • To determine the influence of liposome composition on nanotubule formation and stability.

Main Methods:

  • Surface-immobilized liposomes were subjected to electric fields ranging from 2 to 20 V/cm.
  • Fluorescent microscopy was used to observe and quantify nanotubule formation from labeled phospholipids.
  • Liposome composition, including charged lipids and cholesterol, was varied.

More Related Videos

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes
09:47

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
05:16

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Published on: July 26, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
07:49

Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum

Published on: January 22, 2019

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes
09:47

Facile Preparation of Internally Self-assembled Lipid Particles Stabilized by Carbon Nanotubes

Published on: February 19, 2016

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay
05:16

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Published on: July 26, 2021

Main Results:

  • Stable, millimeter-long nanotubules formed, generally aligning with the electric field.
  • Onset voltages for nanotubule formation ranged from 4 to 15 V/cm, dependent on liposome composition.
  • Increased charged lipid content reduced onset voltage, while >30% cholesterol hindered tubule extension.

Conclusions:

  • Electric field application offers a novel strategy for fabricating liposomal nanotubules.
  • Lipid composition significantly impacts the electric field thresholds and efficiency of nanotubule formation.
  • Lipid migration and domain formation are proposed as key mechanisms for tubule growth.