Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 27, 2026

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

Scaffold-free human cardiac tissue patch created from embryonic stem cells.

Kelly R Stevens1, Lil Pabon, Veronica Muskheli

  • 1Department of Bioengineering, University of Washington, Seattle, Washington, USA.

Tissue Engineering. Part A
|December 10, 2008
PubMed
Summary

Researchers developed a novel method for creating scaffold-free human cardiac tissue patches from stem cells. This breakthrough addresses key challenges in cardiac tissue engineering, paving the way for scalable production of functional myocardial constructs.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Circumferential strain recovery after human cardiomyocyte transplantation in minipigs using a novel frequency-based method for myocardial tagging quantification.

Journal of cardiovascular magnetic resonance : official journal of the Society for Cardiovascular Magnetic Resonance·2026
Same author

3D Printing of Enzymatically Softening Hydrogel Biomaterials.

Regenerative engineering and translational medicine·2026
Same author

Cell-type specific allelic dampening of sex-linked genes in sex chromosome aneuploidy.

bioRxiv : the preprint server for biology·2026
Same author

Nanopatterned Cell Sheet Assembly of Biomimetic Cardiac Laminae for Modeling Structure-Function Relationships.

Biomaterials research·2026
Same author

Reliable Identification of Cardiac Maturation Markers Using Robust and Flexible Label-Free Proteomic Quantitation by Spectral Counting on Relatively Abundant Proteins.

Journal of proteome research·2026
Same author

3D reconstruction of human liver tissue at cellular resolution.

Science advances·2026

Area of Science:

  • Biomedical Engineering
  • Stem Cell Biology
  • Cardiovascular Research

Background:

  • Cardiac tissue engineering faces limitations due to biomaterial responses, limited human cardiomyocyte sources, and scalable fabrication challenges.
  • Existing methods often rely on scaffolds, which can elicit adverse host responses and complicate tissue integration.

Purpose of the Study:

  • To develop a novel, scalable, scaffold-free method for generating human cardiac tissue patches.
  • To overcome limitations in human cell sourcing and tissue fabrication for cardiac repair.

Main Methods:

  • Human embryonic stem cells were differentiated into cardiomyocytes using activin A and BMP4.
  • Cells were cultured in suspension on a rotating orbital shaker to promote aggregation into tissue patches.

More Related Videos

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
08:16

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Published on: November 5, 2016

Related Experiment Videos

Last Updated: Jun 27, 2026

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
06:57

A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

Published on: August 5, 2018

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
06:17

3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells

Published on: March 28, 2025

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies
08:16

Neonatal Cardiac Scaffolds: Novel Matrices for Regenerative Studies

Published on: November 5, 2016

  • Cardiomyocyte purity, maturation, proliferation, and electromechanical coupling were assessed over time.

    • Main Results:

    • Macroscopic, beating, disc-shaped cardiac tissue patches were formed within 2 days.
    • Patch size correlated with cell input, reaching approximately 11 mm in diameter with 12 million cells.
    • Cardiomyocyte purity increased to approximately 80% by day 11, with evidence of automaticity and synchronous calcium transients.

    Conclusions:

    • The developed method provides a scalable, scaffold-free approach for generating functional human cardiac tissue.
    • This technique addresses critical hurdles in human cell sourcing and tissue fabrication for cardiac regenerative medicine.
    • The resulting cardiac patches demonstrate electromechanical coupling, indicating potential for therapeutic applications.