Related Experiment Video
Updated: Jun 27, 2026

08:32
Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Simultaneous widefield single molecule orientation and FRET microscopy in cells
S E D Webb1, D J Rolfe, S R Needham
1Science and Technology Facilities Council, Daresbury Laboratory, Warrington WA4 4AD, UK. stephen.webb@stfc.ac.uk
Optics Express
|December 10, 2008
Abstract:
We combine single molecule fluorescence orientation imaging with single-pair fluorescence resonance energy transfer microscopy, using a total internal reflection microscope. We show how angles and FRET efficiencies can be determined for membrane proteins at the single molecule level and provide data from the epidermal growth factor receptor system in cells.

