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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 27, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Multi-photon microscopy with a low-cost and highly efficient Cr:LiCAF laser.

Sava Sakadzić1, Umit Demirbas, Thorsten R Mempel

  • 1MGH/MIT/HMS Athinuola A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital/Harvard Medical School, Charlestown, Massachusetts 02129, USA.

Optics Express
|December 10, 2008
PubMed
Summary

A new, low-cost Cr:LiCAF laser offers an alternative to expensive Ti:Sapphire lasers for multi-photon microscopy (MPM). This technology enables advanced cellular imaging in various biomedical fields, making MPM more accessible.

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Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

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Last Updated: Jun 27, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy
08:48

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

Area of Science:

  • Biomedical Imaging
  • Laser Technology
  • Microscopy

Background:

  • Multi-photon microscopy (MPM) provides high-resolution cellular and subcellular imaging.
  • High cost and complexity of femtosecond lasers limit widespread MPM adoption.

Purpose of the Study:

  • To introduce a low-cost femtosecond diode-pumped Cr:LiCAF laser as an alternative to Ti:Sapphire lasers for MPM.
  • To demonstrate the efficacy of this Cr:LiCAF laser in diverse MPM applications.

Main Methods:

  • Development of a diode-pumped Cr:LiCAF laser system using inexpensive single-mode pump diodes.
  • Characterization of laser output: ~70-fs pulses, 1.8-nJ energy, 800-nm wavelength, 100-MHz repetition rate, 180-mW average power.

Main Results:

  • The Cr:LiCAF laser successfully generated femtosecond pulses suitable for MPM.
  • Demonstrated MPM imaging capabilities in neuroscience, immunology, endocrinology, and cancer research.
  • Achieved comparable imaging quality to traditional MPM systems.

Conclusions:

  • Femtosecond diode-pumped Cr:LiCAF laser technology presents a cost-effective solution for MPM.
  • This laser source has broad applicability across multiple biomedical research areas.
  • Increased accessibility of MPM technology for scientific research.